Catch and Release: Integrated System for Multiplexed Detection of Bacteria

详细信息    查看全文

• 作者：Jasenka Verbarg ; William D. Plath ; Lisa C. Shriver-Lake ; Peter B. Howell ; Jr. ; Jeffrey S. Erickson ; Joel P. Golden ; Frances S. Ligler
• 刊名：Analytical Chemistry
• 出版年：2013
• 出版时间：May 21, 2013
• 年：2013
• 卷：85
• 期：10
• 页码：4944-4950
• 全文大小：267K
• 年卷期：v.85,no.10(May 21, 2013)
• ISSN：1520-6882

文摘

An integrated system with automated immunomagnetic separation and processing of fluidic samples was demonstrated for multiplexed optical detection of bacterial targets. Mixtures of target-specific magnetic bead sets were processed in the NRL MagTrap with the aid of rotating magnet arrays that entrapped and moved the beads within the channel during reagent processing. Processing was performed in buffer and human serum matrixes with 10-fold dilutions in the range of 10²鈥?0⁶ cells/mL of target bacteria. Reversal of magnets鈥?rotation post-processing released the beads back into the flow and moved them into the microflow cytometer for optical interrogation. Identification of the beads and the detection of PE fluorescence were performed simultaneously for multiplexed detection. Multiplexing was performed with specifically targeted bead sets to detect E. coli 0157.H7, Salmonella Common Structural Antigen, Listeria sp., and Shigella sp., dose鈥搑esponse curves were obtained, and limits of detection were calculated for each target in the buffer and clinical matrix. Additional tests demonstrated the potential for using the MagTrap to concentrate target from larger volumes of sample prior to the addition of assay reagents.