Embryonic Sex Induces Differential Expression of Proteins in Bovine Uterine Fluid

详细信息    查看全文

• 作者：Enrique G贸mez ; Jos茅 N. Caama帽o ; Fernando J. Corrales ; Carmen D铆ez ; Eva Correia-脕lvarez ; David Mart铆n ; Beatriz Trigal ; Susana Carrocera ; Mar铆a I. Mora ; Jairo Pello-Palma ; Juan F. Moreno ; Marta Mu帽oz
• 刊名：Journal of Proteome Research
• 出版年：2013
• 出版时间：March 1, 2013
• 年：2013
• 卷：12
• 期：3
• 页码：1199-1210
• 全文大小：471K
• 年卷期：v.12,no.3(March 1, 2013)
• ISSN：1535-3907

文摘

The bovine endometrium recognizes early embryos and reacts differently depending on the developmental potential of the embryo. However, it is unknown whether the endometrium can distinguish embryonic sex. Our objective was to analyze sexual dimorphism in the uterus in response to male and female embryos. Differentially expressed (DE) proteins, different levels of hexoses, and other embryotrophic differences were analyzed in uterine fluid (UF). Proteomic analysis of day-8 UF recovered from heifers after the transfer of day-5 male or female embryos identified 23 DE proteins. Regulated proteasome/immunoproteasome protein subunits indicated differences in antigen processing between UF carrying male embryos (male-UF) or female embryos (female-UF). Several enzymes involved in glycolysis/gluconeogenesis and antioxidative/antistress responses were up-regulated in female-UF. Fructose concentration was increased in female-UF versus male-UF, while glucose levels were similar. In vitro cultures with molecules isolated from male-UF were found to improve male embryo development compared to female embryos cultured with molecules isolated from female-UF. We postulated that, in vivo, male embryos induce changes in the endometrium to help ensure their survival. In contrast, female embryos do not appear to induce these changes.

Keywords:

bovine; embryo; sex; uterus; dimorphism; proteomics