High Redox Potential Cathode Based on Laccase Covalently Attached to Gold Electrode

详细信息    查看全文

• 作者：Marcos Pita ; Cristina Gutierrez-Sanchez ; David Olea ; Marisela Velez ; Cristina Garcia-Diego ; Sergey Shleev ; Victor M. Fernandez ; Antonio L. De Lacey
• 刊名：Journal of Physical Chemistry C
• 出版年：2011
• 出版时间：July 14, 2011
• 年：2011
• 卷：115
• 期：27
• 页码：13420-13428
• 全文大小：1011K
• 年卷期：v.115,no.27(July 14, 2011)
• ISSN：1932-7455

文摘

A new strategy for oriented covalent immobilization of Trametes hirsuta laccase on gold electrodes is presented. The strategy is based on the gold surface modification with a mixed monolayer of an aromatic diazonium salt derivative and 6-mercapto-1-hexanol for further use as scaffold for the enzyme鈥檚 covalent linkage. This strategy offers a variety of advantages such as high stability and laccase-friendly support morphology, which turns it into a suitable metal鈥揺nzyme interface. Conditions aiming at optimum orientation for direct electron transfer (DET) via the T1 copper site were studied. Current density values up to 40 渭A路cm^鈥? were measured for the electrocatalytic reduction of O₂ in the absence of redox mediators. This strategy is a big step forward in the development of laccase-modified gold electrodes for bioelectrocatalytic reduction of O₂.