New Generation of Bioorthogonally Applicable Fluorogenic Dyes with Visible Excitations and Large Stokes Shifts

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• 作者：Andr谩s Herner ; Gemma Estrada Girona ; Ivana Niki膰 ; Mih谩ly K谩llay ; Edward A. Lemke ; P茅ter Kele
• 刊名：Bioconjugate Chemistry
• 出版年：2014
• 出版时间：July 16, 2014
• 年：2014
• 卷：25
• 期：7
• 页码：1370-1374
• 全文大小：272K
• ISSN：1520-4812

文摘

Synthesis of a set of new, azide bearing, biorthogonally applicable fluorogenic dyes with large Stokes shifts is presented herein. To assess the fluorogenic performance of these new dyes we have labeled a genetically modulated, cyclooctyne-bearing protein in lysate medium. Studies showed that the labels produce specific signal with minimal background fluorescence. We also provide theoretical insights into the design of such fluorogenic labels.