Characterization of Nucleosides and Nucleobases in Fruits of Ziziphus jujuba by UPLC-DAD-MS
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- 作者：Sheng Guo ; Jin-Ao Duan ; Yu-Ping Tang ; Zhen-Hua Zhu ; Ye-Fei Qian ; Nian-Yun Yang ; Er-Xin Shang ; Da-Wei Qian
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2010
- 出版时间：October 13, 2010
- 年：2010
- 卷：58
- 期：19
- 页码：10774-10780
- 全文大小：791K
- 年卷期：v.58,no.19(October 13, 2010)
- ISSN：1520-5118
文摘
The fruit of Ziziphus jujuba, named dazao in Chinese, has been utilized as food as well as crude drugs in China for thousands of years. To explore the profiles of the nucleosides and nucleobases in this fruit, an ultraperformance liquid chromatograph coupled with a photodiode array detector and electrospray ionization−mass spectrometer method (UPLC-DAD-MS) has been established and validated in this paper. The validated method was successfully applied for the simultaneous characterization and quantitation of 9 nucleosides and nucleobases in 49 dazao samples, which comprised 43 cultivars from 26 cultivation regions. Furthermore, principal component analysis (PCA) was performed to classify the samples on the basis of the contents of the nine analyzed compounds. The results showed that almost all of these dazao samples were rich in nucleosides and nucleobases, although their contents were obviously various, and the proposed method could serve as a prerequisite for quality control of jujube products.