Rapid Determination of Amino Acids in Fruits of Ziziphus jujubaby Hydrophilic Interaction Ultra-High-Performance Liquid Chromatography Coupled with Triple-Quadrupole Mass Spectrometry
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- 作者：Sheng Guo ; Jin-ao Duan ; Dawei Qian ; Yuping Tang ; Yefei Qian ; Dawei Wu ; Shulan Su ; Erxin Shang
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2013
- 出版时间：March 20, 2013
- 年：2013
- 卷：61
- 期：11
- 页码：2709-2719
- 全文大小：330K
- 年卷期：v.61,no.11(March 20, 2013)
- ISSN：1520-5118
文摘
In this study, a sensitive and rapid method for the simultaneous determination of free amino acids without derivatization using hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry (HILIC鈥揗S/MS) was developed. The method was performed on an ultra-high-performance liquid chromatography (UHPLC) separation system coupled with a triple-quadrupole mass spectrometry (TQ-MS) instrument. Sufficient separation of 23 underivatized amino acids was achieved on an Acquity BEH Amide column (2.1 mm 脳 100 mm, 1.7 渭m) in a single run of 12 min. Then the method was applied for the analysis of the free amino acids in 46 batches of Ziziphus jujuba fruits which comprised 39 cultivars from 26 cultivation regions. Multivariate statistical analysis was also used to investigate the differences in free amino acid profiles among the samples. This study showed that HILIC鈥揢HPLC鈥揟Q-MS is an effective technique to analyze underivatized amino acids in the food samples.