Influence of the Length of Imogolite-Like Nanotubes on Their Cytotoxicity and Genotoxicity toward Human Dermal Cells

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• 作者：Wei Liu ; Perrine Chaurand ; Carole Di Giorgio ; Michel De M茅o ; Antoine Thill ; M茅lanie Auffan ; Armand Masion ; Daniel Borschneck ; Florence Chaspoul ; Philippe Gallice ; Alain Botta ; Jean-Yves Bottero ; J茅r么me Rose
• 刊名：Chemical Research in Toxicology
• 出版年：2012
• 出版时间：November 19, 2012
• 年：2012
• 卷：25
• 期：11
• 页码：2513-2522
• 全文大小：455K
• 年卷期：v.25,no.11(November 19, 2012)
• ISSN：1520-5010

文摘

Physical鈥揷hemical parameters such as purity, structure, chemistry, length, and aspect ratio of nanoparticles (NPs) are linked to their toxicity. Here, synthetic imogolite-like nanotubes with a set chemical composition but various sizes and shapes were used as models to investigate the influence of these physical parameters on the cyto- and genotoxicity and cellular uptake of NPs. The NPs were characterized using X-ray diffraction (XRD), small angle X-ray scattering (SAXS), and atomic force microscopy (AFM). Imogolite precursors (PR, ca. 5 nm curved platelets), as well as short tubes (ST, ca. 6 nm) and long tubes (LT, ca. 50 nm), remained stable in the cell culture medium. Internalization into human fibroblasts was observed only for the small particles PR and ST. None of the tested particles induced a significant cytotoxicity up to a concentration of 10^鈥? mg路mL^鈥?. However, small sized NPs (PR and ST) were found to be genotoxic at very low concentration 10^鈥? mg路mL^鈥?, while LT particles exhibited a weak genotoxicity. Our results indicate that small size NPs (PR, ST) were able to induce primary lesions of DNA at very low concentrations and that this DNA damage was exclusively induced by oxidative stress. The higher aspect ratio LT particles exhibited a weaker genotoxicity, where oxidative stress is a minor factor, and the likely involvement of other mechanisms. Moreover, a relationship among cell uptake, particle aspect ratio, and DNA damage of NPs was observed.