Bioactivity Profiling with Parallel Mass Spectrometry Reveals an Assemblage of Green Tea Metabolites Affording Protection against Human Huntingtin and α-Synuclein Toxicity
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- 作者：Russell B. Williams ; Will R. Gutekunst ; P. Matthew Joyner ; Wenzhen Duan ; Qing Li ; Christopher A. Ross ; Todd D. Williams ; Robert H. Cichewicz
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2007
- 出版时间：November 14, 2007
- 年：2007
- 卷：55
- 期：23
- 页码：9450 - 9456
- 全文大小：194K
- 年卷期：v.55,no.23(November 14, 2007)
- ISSN：1520-5118
文摘
Aberrant protein aggregation and misfolding are key pathological features of many neurodegenerative disorders, including Huntington’s and Parkinson’s diseases. Compounds that offer protection from toxicity associated with aggregation-prone neurodegenerative proteins may have applications for the treatment of a multitude of disorders. A high-throughput bioassay system with parallel electrospray ionization mass spectrometry screening has been designed for critical evaluation of milligram quantities of natural product extracts, including dietary substances, for compounds of pharmacological relevance to the treatment of human neurodegenerative diseases. Using Saccharomyces cerevisiae strains engineered to express mutant human huntingtin and α-synuclein, we are able to identify extracts and compounds that protect cells from toxicity associated with these proteins. Applying this screening paradigm, we determined that a bioactive green tea extract contains an assemblage of catechins that were individually characterized for their respective protective effects against huntingtin and α-synuclein toxicity.