Gene Silencing by siRNA Microhydrogels via Polymeric Nanoscale Condensation

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• 作者：Cheol Am Hong ; Soo Hyeon Lee ; Jee Seon Kim ; Ji Won Park ; Ki Hyun Bae ; Hyejung Mok ; Tae Gwan Park ; Haeshin Lee
• 刊名：Journal of the American Chemical Society
• 出版年：2011
• 出版时间：September 7, 2011
• 年：2011
• 卷：133
• 期：35
• 页码：13914-13917
• 全文大小：783K
• 年卷期：v.133,no.35(September 7, 2011)
• ISSN：1520-5126

文摘

The first attempt to prepare biologically active siRNA-based microhydrogels is reported. The self-assembled microhydrogels were fabricated using sense/antisense complementary hybridization between single-stranded linear and Y-shaped trimeric siRNAs. The siRNA microhydrogels were condensed using a popular cationic polymer such as LPEI to form compact, stable siRNA/polymeric nanoparticles that exhibited superb cellular uptake efficiency and gene silencing activity.