Development of an Aerosol Chemiluminescent Detector Coupled to Capillary Electrophoresis for Saccharide Analysis
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- 作者：Guangming Huang ; Yi Lv ; Sichun Zhang ; Chengdui Yang ; and Xinrong Zhang
- 刊名：Analytical Chemistry
- 出版年：2005
- 出版时间：November 15, 2005
- 年：2005
- 卷：77
- 期：22
- 页码：7356 - 7365
- 全文大小：358K
- 年卷期：v.77,no.22(November 15, 2005)
- ISSN：1520-6882
文摘
A novel aerosol chemiluminescent (CL) detector couplingto capillary electrophoresis (CE) for the detection ofsaccharides is reported. This CL detector is composed ofa postcapillary nebulizer and porous alumina as catalyzerin quartz tube. The CL emission could be generated dueto the catalyzing oxidization of saccharides on the surfaceof porous alumina. The saccharides such as sucrose,
-lactose, maltose, raffinose, galactose, xylose, and glucose with only weak UV absorbance can be successfullydetected. The linear ranges of those saccharides are from30-2000 to 50-2000 mg/L; relative standard deviationsrange from 2.1 to 3.7% (200 mg/L, n = 11). Comparedwith the traditional UV detector currently used in CE, thisnovel detector shows the advantage of high sensitivity tothe compounds with only weak UV absorption. Thus, itcould be an important supplement of CE detectors for UV-lacking compounds.
-lactose, maltose, raffinose, galactose, xylose, and glucose with only weak UV absorbance can be successfullydetected. The linear ranges of those saccharides are from30-2000 to 50-2000 mg/L; relative standard deviationsrange from 2.1 to 3.7% (200 mg/L, n = 11). Comparedwith the traditional UV detector currently used in CE, thisnovel detector shows the advantage of high sensitivity tothe compounds with only weak UV absorption. Thus, itcould be an important supplement of CE detectors for UV-lacking compounds.