Relative Inhibition of Lipid Peroxidation, Cyclooxygenase Enzymes, and Human Tumor Cell Proliferation by Natural Food Colors
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- 作者：Muntha K. Reddy ; Ruby L. Alexander-Lindo ; and Muraleedharan G. Nair
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2005
- 出版时间：November 16, 2005
- 年：2005
- 卷：53
- 期：23
- 页码：9268 - 9273
- 全文大小：224K
- 年卷期：v.53,no.23(November 16, 2005)
- ISSN：1520-5118
文摘
The most abundant water soluble natural food colors are betacyanins and anthocyanins. Similarly,lycopene, bixin, 
le">-carotene, and chlorophyll are water insoluble colors. Pure betanin, bixin, lycopene,chlorophyll, le">-carotene, and cyanidin-3-O-glucoside were isolated from Beta vulgaris, Bixa orellana,Lycopersicum esculentum, Spinacia oleracea, Daucus carrota, and Prunus cerasus, respectively.These natural pigments, alone and in combination, were evaluated for their relative potencies againstcyclooxygenase enzymes and tumor cell growth inhibition by using MCF-7 (breast), HCT-116 (colon),AGS (stomach), CNS (central nervous system), and NCI-H460 (lung) tumor cell lines. Among thecolors tested, betanin, cyanidin-3-O-glucoside, lycopene, and le">-carotene inhibited lipid peroxidation.However, all pigments tested gave COX-1 and COX-2 inhibition and showed a dose-dependent growthinhibition against breast, colon, stomach, central nervous system, and lung tumor cells, respectively.The mixtures of these pigments were also evaluated for their synergistic effects and chemicalinteractions at various concentrations. The mixture of anthocyanin and betanin negated their efficacyin the cell growth inhibitory assay and did not enhance the COX enzyme inhibitory activity. This isthe first report of a comparative evaluation and the impact on biological activities of these pigmentsalone and in combination.Keywords: Betanin; cyanidin-3-O-glucoside; lycopene; bixin; le">-carotene; chlorophyll; cell proliferationinhibition; antioxidant; COX-1; COX-2
le">-carotene, and chlorophyll are water insoluble colors. Pure betanin, bixin, lycopene,chlorophyll, le">-carotene, and cyanidin-3-O-glucoside were isolated from Beta vulgaris, Bixa orellana,Lycopersicum esculentum, Spinacia oleracea, Daucus carrota, and Prunus cerasus, respectively.These natural pigments, alone and in combination, were evaluated for their relative potencies againstcyclooxygenase enzymes and tumor cell growth inhibition by using MCF-7 (breast), HCT-116 (colon),AGS (stomach), CNS (central nervous system), and NCI-H460 (lung) tumor cell lines. Among thecolors tested, betanin, cyanidin-3-O-glucoside, lycopene, and le">-carotene inhibited lipid peroxidation.However, all pigments tested gave COX-1 and COX-2 inhibition and showed a dose-dependent growthinhibition against breast, colon, stomach, central nervous system, and lung tumor cells, respectively.The mixtures of these pigments were also evaluated for their synergistic effects and chemicalinteractions at various concentrations. The mixture of anthocyanin and betanin negated their efficacyin the cell growth inhibitory assay and did not enhance the COX enzyme inhibitory activity. This isthe first report of a comparative evaluation and the impact on biological activities of these pigmentsalone and in combination.Keywords: Betanin; cyanidin-3-O-glucoside; lycopene; bixin; le">-carotene; chlorophyll; cell proliferationinhibition; antioxidant; COX-1; COX-2