Photocatalytic Regeneration of Nicotinamide Cofactors by Quantum Dot–Enzyme Biohybrid Complexes

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• 作者：Katherine A. Brown ; Molly B. Wilker ; Marko Boehm ; Hayden Hamby ; Gordana Dukovic ; Paul W. King
• 刊名：ACS Catalysis
• 出版年：2016
• 出版时间：April 1, 2016
• 年：2016
• 卷：6
• 期：4
• 页码：2201-2204
• 全文大小：295K
• ISSN：2155-5435

文摘

We report the characterization of biohybrid complexes of CdSe quantum dots and ferredoxin NADP⁺-reductase for photocatalytic regeneration of NADPH. Illumination with visible light led to reduction of NADP⁺ to NADPH, with an apparent k_cat of 1400 h^–1. Regeneration of NADPH was coupled to reduction of aldehydes to alcohols catalyzed by a NADPH-dependent alcohol dehydrogenase, with each NADPH molecule recycled an average of 7.5 times. The quantum yield both of NADPH and alcohol production were 5–6% for both products. Light-driven NADPH regeneration was also demonstrated in a multienzyme system, showing the capacity of QD-FNR complexes to drive continuous NADPH-dependent transformations.