Purification and Characterization of 伪-Galactosidase from White Chickpea (Cicer arietinum)
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- 作者：Neelesh Singh ; Arvind M. Kayastha
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2012
- 出版时间：March 28, 2012
- 年：2012
- 卷：60
- 期：12
- 页码：3253-3259
- 全文大小：288K
- 年卷期：v.60,no.12(March 28, 2012)
- ISSN：1520-5118
文摘
Glycosylated 伪-galactosidase (melibiase) has been purified from white chickpea (Cicer arietinum) to 340-fold with a specific activity of 61 units/mg. Cicer 伪-galactosidase showed a Mr of 45 kDa on SDS-PAGE and by MALDI-TOF. The optimum pH and temperature with pNPGal were 4.5 and 50 掳C, respectively. The Km for hydrolysis of pNPGal was 0.70 mM. Besides hydrolyzing the pNPGal, Cicer 伪-galactosidase also hydrolyzed natural substrates such as melibiose, raffinose, and stachyose very effectively; hence, it can be exploited commercially for improving the nutritional value of soy milk. Galactose was found to be a competitive inhibitor. The property of this enzyme to cleave the terminal galactose residues can be utilized for converting the group B erythrocytes to group O erythrocytes.