Total Cellular RNA Modulates Protein Activity
详细信息 查看全文
- 作者：Subhabrata Majumder ; Christopher M. DeMott ; Sergey Reverdatto ; David S. Burz ; Alexander Shekhtman
- 刊名：Biochemistry
- 出版年：2016
- 出版时间：August 16, 2016
- 年：2016
- 卷：55
- 期：32
- 页码：4568-4573
- 全文大小：350K
- 年卷期：0
- ISSN：1520-4995
文摘
RNA constitutes up to 20% of a cell’s dry weight, corresponding to ∼20 mg/mL. This high concentration of RNA facilitates low-affinity protein–RNA quinary interactions, which may play an important role in facilitating and regulating biological processes. In the yeast Pichia pastoris, the level of ubiquitin-RNA colocalization increases when cells are grown in the presence of dextrose and methanol instead of methanol as the sole carbon source. Total RNA isolated from cells grown in methanol increases β-galactosidase activity relative to that seen with RNA isolated from cells grown in the presence of dextrose and methanol. Because the total cellular RNA content changes with growth medium, protein–RNA quinary interactions can alter in-cell protein biochemistry and may play an important role in cell adaptation, critical to many physiological and pathological states.