Versatile Microfluidic Platform for the Assessment of Sialic Acid Expression on Cancer Cells Using Quantum Dots with Phenylboronic Acid Tags

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• 作者：Jun-Tao Cao ; Peng-Hui Zhang ; Yan-Ming Liu ; E. S. Abdel-Halim ; Jun-Jie Zhu
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2015
• 出版时间：July 15, 2015
• 年：2015
• 卷：7
• 期：27
• 页码：14878-14884
• 全文大小：422K
• ISSN：1944-8252

文摘

This work describes a versatile microfluidic platform for evaluation of cell-surface glycan expression at the single-cell level using quantum dots (QDs) tagged with phenylboronic acid. The platform was integrated with dual microwell arrays, allowing the introduction of cells in two states using the same cell culture chamber. The simultaneous analysis of cells in the same environment minimized errors resulting from different culture conditions. As proof-of-concept, the expressions of sialic acid (SA) groups on K562 cells, with or without 3鈥?azido-3鈥?deoxythymidine (AZT) treatment, were evaluated in the same chamber. 3-Aminophenylboronic acid functionalized CdSeTe@ZnS-SiO<sub>2sub> QDs (APBA-QDs) were prepared as probes to recognize SA groups on K562 cells with only one-step labeling. The results showed that the expression of SA moieties on K562 cells was increased by 18% and 31% after treatment with 20 and 40 渭M AZT, respectively. Performing the drug treatment and control experiments simultaneously in the same chamber significantly improved the robustness and effectiveness of the assay. The strategy presented here provides an alternative tool for glycan analysis in a sensitive, high-throughput, and effective manner.