Optical Biosensor Based On Hollow Integrated Waveguides
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- 作者：Ví ; ctor J. Cadarso ; Cé ; sar Ferná ; ndez-Sá ; nchez ; Andreu Llobera ; Margarita Darder ; Carlos Domí ; nguez
- 刊名：Analytical Chemistry
- 出版年：2008
- 出版时间：May 1, 2008
- 年：2008
- 卷：80
- 期：9
- 页码：3498 - 3501
- 全文大小：136K
- 年卷期：v.80,no.9(May 1, 2008)
- ISSN：1520-6882
文摘
The first absorbance biosensor based on pure siliconhollow integrated waveguides is presented in this work.With the use of horseradish peroxidase (HRP) as a modelrecognition element, an enzymatic sensor for the measurement of hydrogen peroxide was fabricated, numerically simulated, and experimentally characterized. Waveguideswith widths ranging from 50 to 80 
m, having a depth of50 m and lengths up to 5 mm were easily fabricated byjust one photolithographic step. These were further modified by covalent immobilization of HRP using silanizationchemistry. Simulation studies of the proposed approachshowed a sensor linear behavior up to 300 M H2O2 anda sensitivity of 2.7 × 10-3 AU/M. Experimental resultswere in good agreement with the simulated ones. A linearbehavior between 10 and 300 M H2O2, a sensitivity of3 × 10-3 AU/M, and a signal-to-noise ratio around 20dB were attained. Also, kinetic studies of the activity ofthe immobilized enzyme on the silicon waveguide surfacegave an apparent Michaelis-Menten constant of 0.44mM. The simple technology proposed in this work enablesthe fabrication of cost-effective, easy-to-use, miniaturizedbiosensor generic platforms, these being envisioned asexcellent candidates for the development of lab-on-a-chipsystems.
m, having a depth of50 m and lengths up to 5 mm were easily fabricated byjust one photolithographic step. These were further modified by covalent immobilization of HRP using silanizationchemistry. Simulation studies of the proposed approachshowed a sensor linear behavior up to 300 M H2O2 anda sensitivity of 2.7 × 10-3 AU/M. Experimental resultswere in good agreement with the simulated ones. A linearbehavior between 10 and 300 M H2O2, a sensitivity of3 × 10-3 AU/M, and a signal-to-noise ratio around 20dB were attained. Also, kinetic studies of the activity ofthe immobilized enzyme on the silicon waveguide surfacegave an apparent Michaelis-Menten constant of 0.44mM. The simple technology proposed in this work enablesthe fabrication of cost-effective, easy-to-use, miniaturizedbiosensor generic platforms, these being envisioned asexcellent candidates for the development of lab-on-a-chipsystems.