Immunophenotyping and characterization of BNP colostra revealed pathogenic alloantibodies of IgG1 subclass with specifity to platelets, granulocytes and monocytes of all maturation stages

详细信息    查看全文

• 作者：Aryan Assad^a ; ^{a.assad@tiph.vetmed.uni-muenchen.de} ; Barbara Amann^b ; ^{baerbl.amann@tiph.vetmed.uni-muenchen.de} ; Annette Friedrich^a ; ^{a.friedrich@lmu.de} ; Cornelia Annette Deeg^b ; ^{deeg@tiph.vetmed.uni-muenchen.de}
• 关键词：Bovine neonatal pancytopenia ; Colostrum ; IgG1 alloantibodies ; Subclass ; Platelets ; Myeloid lineage
• 刊名：Veterinary Immunology and Immunopathology
• 出版年：2012
• 出版时间：15 June, 2012
• 年：2012
• 卷：147
• 期：1-2
• 页码：25-34
• 全文大小：934 K

文摘

Bovine neonatal pancytopenia (BNP) is mainly characterized by multiple haemorrhages, thrombocytopenia and leukocytopenia as a result of bone marrow depletion. BNP can be induced in healthy calves through application of colostrum from BNP donors, proofing that BNP is mediated to maternal alloantibodies. Alloantibody binding to bovine blood cells is present in sera and colostra of BNP donors and is probably initialized by vaccination with a certain BVD vaccine. To understand etiology and pathomechanisms of BNP, we closely characterized disease inducing antibodies regarding immunoglobulin subclass and binding specificities to peripheral blood derived leukocytes and platelets. By exact phenotyping the targeted blood cell subsets, including platelets for the first time, we investigated that BNP alloantibodies are exclusively of IgG1 subclass. Interestingly, IgG1 of BNP colostra bound to 70 % leukocytes and 100 % platelets irrespective of different bovine breeds and cellular maturity of all specimens tested. Furthermore, staining pattern on platelets as well as leukocyte subsets by BNP-IgG1 alloantibody exposed 100 % reactivity to platelets, granulocytes and monocytes. Interestingly, the main part of T-helper cells was not bound by colostral alloantibodies. Our results point to a crucial role of IgG1 antibodies in BNP and to a target antigen that is expressed by all cells of myeloid lineage, but only partially by the lymphoid lineage.