文摘
A novel fibrinolytic enzyme was purified from food-grade fungus, Neurospora sitophila. It is a monomer with molecular mass of 34 kDa; and has pI of 9.3 ± 0.2 as determined by IEF. It was optimally active at pH 7.6 and 41 °C; stable up to 52 °C and in wide pH 4–11. It hydrolyzed fibrin clot directly, and cleaved Aα, Bβ and γ-chains of fibrinogen. It can be developed as a safe potential candidate for oral fibrinolytic therapy.