Survey of five food-borne pathogens in commercial cold food dishes and their detection by multiplex PCR
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- 作者：Qian Yu ; Ligong Zhai ; Xiaomei Bie ; bxm43@njau.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Zhaoxin Lu ; Chong Zhang ; Tingting Tao ; Junjie Li ; Fengxia Lv ; Haizhen Zhao
- 关键词：Cold dishes ; Market survey of pathogens ; Conventional culture method ; Multiplex PCR assay
- 刊名：Food Control
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：59
- 期：Complete
- 页码：862-869
- 全文大小：464 K
文摘
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The market survey showed that cold dishes were contaminated by Staphylococcus aureus, Salmonella spp. and Listeria monocytogenes.
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A rapid multiplex PCR assay was developed for three pathogens.
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The assay was 100% specific across 86 strains.
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The assay was sensitive with a detection limit of 103 cfu/ml for pure cultures.
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The practical sample testing by multiplex PCR was satisfactory.