Nitric oxide regulates Angiopoietin1/Tie2 expression after stroke
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- 作者：Alex Zacharek ; Jieli Chen ; Chunling Zhang ; Xu Cui ; Cynthia Roberts ; Hao Jiang ; Hua Teng and Michael Chopp
- 关键词：Angiogenesis ; DETA-NONOate ; Angiopoietin1 ; Tie2 ; Occludin ; Stroke
- 刊名：Neuroscience Letters
- 出版年：2006
- 出版时间：14 August 2006
- 年：2006
- 卷：404
- 期：1-2
- 页码：28-32
- 全文大小：370 K
文摘
We tested whether the nitric oxide donor, (Z)-1-[N-(2-aminoethyl)-N-(2-ammonioethyl) aminio] diazen-1-ium-1,2-diolate (DETA-NONOate), increases expression of Angiopoietin (Ang1)/Tie2, which may play a role in regulating angiogenesis and vascular integrity after stroke in rats. Wistar rats were subjected to middle cerebral artery occlusion and treated with or without DETA-NONOate. Stroke rats treated with DETA-NONOate show significantly increased Ang1, Tie2 and Occludin expression in the ischemic border compared with control stroke animals (p < 0.05). Consistent with in vivo data, DETA-NONOate promotes capillary tube formation in cultured brain endothelial cells. Neutralizing Ang1 antibody attenuates DETA-NONOate-induced capillary tube formation. The data suggest that the Ang1/Tie2 axis promotes DETA-NONOate-induced angiogenesis and stabilizes of angiogenic vessels after stroke.