Characterization of a single-chain variable fragment specific to Cronobacter spp. from hybridoma based on outer membrane protein A
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- 作者：Qiming Chena ; Tingting Taoa ; Xiaomei Biea ; Fengxia Lua ; Yuanhong Lia ; b ; Zhaoxin Lua ; fmb@njau.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Cronobacter spp. ; OmpA ; ScFv ; Bioinformatics analysis
- 刊名：Journal of Microbiological Methods
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：129
- 期：Complete
- 页码：136-143
- 全文大小：921 K
文摘
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The protein sequence of OmpA of Cronobacter spp. were analyzed.
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A scFv based on protein OmpA specific to Cronobacter spp. was constructed.
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The whole preparation process of scFvH81 only required five days.