Determination of aglycone conjugated metabolites of scutellarin in rat plasma by HPLC

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• 作者：Jianming Huang ; Ni Li ; Yunqiu Yu ; Weiyu Weng and Xubing Huang
• 关键词：Scutellarin ; Scutellarein ; HPLC determination
• 刊名：Journal of Pharmaceutical and Biomedical Analysis
• 出版年：2006
• 出版时间：13 February 2006
• 年：2006
• 卷：40
• 期：2
• 页码：465-471
• 全文大小：248 K

文摘

A specific, precise and accurate high-performance liquid chromatography (HPLC) method for the determination of aglycone conjugated metabolites of scutellarin in plasma after enzymolysis to scutellarein (the aglycone of scutellarin) was developed and validated. The chromatographic separation was performed on a Lunar C₁₈(2) reversed-phase column at a column temperature of 40 °C. The mobile phase, delivered at 1.0 ml/min, consisted of acetonitrile–KH₂PO₄ buffer (40 mM, pH 2.5) (33:67, v/v). The detection wavelength was set at 335nm. Scutellarein and I.S. (quercetin) were isolated by a liquid–liquid extraction after incubating the plasma samples with β-glucuronidase/sulfatase. The method was validated using scutellarin spiked plasma as standards. Linearity was confirmed in the concentration range of 0.2165–4.329 nmol/ml, R.S.D.s were within 8.32 % , and the recoveries of scutellarein ranged from 101.2 to 108.6 % . The method is applicable to the pharmacokinetic study of aglycone conjugated metabolites of scutellarin in rats after oral administration of scutellarin.