Genetic Variations of the Parasitic Dinoflagellate Hematodinium Infecting Cultured Marine Crustaceans in China

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• 作者：Jie Xiao^a ; ^c ; Xiaoxiang Miao^a ; Caiwen Li^b ; ^c ; ¹ ; ^{cwli@qdio.ac.cn} ; ^{caiwenli@live.com} ; Wenjun Xu^d ; Xuelei Zhang^a ; ^c ; Zongling Wang^a ; ^c
• 关键词：Crustacean ; parasitic dinoflagellate ; molecular diversity ; phylogenetic structure ; ribosome RNA ; haplotype.
• 刊名：Protist
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：167
• 期：6
• 页码：597-609
• 全文大小：756 K
• 卷排序：167

文摘

The parasitic dinoflagellate Hematodinium infects multiple cultured marine crustaceans and has resulted in significant economic losses to their aquaculture in China. Limited molecular data implied a close relationship among Hematodinium reported in China, whereas the genetic diversity and detailed genetic variation within Hematodinium remains unclear. In order to investigate the genetic diversity and composition of the parasitic dinoflagellate in China, the sequences of the internal transcribed spacer region (ITS1 – 5.8S – ITS2) were amplified from 104 infected hosts cultured in three geographic locations. Two strains were identified, Hematodinium perezi II was the prevalent strain observed in all three host taxa throughout the survey, the other novel one was detected from the single Exopalaemon carinicauda collected from Zhejiang province. Eight ITS haplotypes within H. perezi II were identified with a similarity of 99.8% –100%. The distinct haplotype compositions and AMOVA analysis suggested a significant genetic differentiation and population structure among the three geographic populations of H. perezi II. The novel strain, genetically affiliated with H. perezi, was quite divergent from the three known genotypes (I, II and III) of H. perezi, and further investigation is needed to determine the distribution and host range of this new strain.