The crystal structure of HIV CRF07 B¡ä/C gp41 reveals a hyper-mutant site in the middle of HR2 heptad repeat
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- 作者：Jiansen Du ; Hailing Xue ; Jing Ma ; Fang Liu ; Jianhua Zhou ; Yiming Shao ; Wentao Qiao ; Xinqi Liu
- 关键词：HIV ; gp41 ; Crystal structure ; Membrane fusion ; Heptad repeat
- 刊名：Virology
- 出版年：2013
- 出版时间：November, 2013
- 年：2013
- 卷：446
- 期：1-2
- 页码：86-94
- 全文大小：5024 K
文摘
HIV CRF07 B¡ä/C is a strain circulating mainly in northwest region of China. The gp41 region of CRF07 is derived from a clade C virus. In order to compare the difference of CRF07 gp41 with that of typical clade B virus, we solved the crystal structure of the core region of CRF07 gp41. Compared with clade B gp41, CRF07 gp41 evolved more basic and hydrophilic residues on its helix bundle surface. Based on sequence alignment, a hyper-mutant cluster located in the middle of HR2 heptads repeat was identified. The mutational study of these residues revealed that this site is important in HIV mediated cell-cell fusion and plays critical roles in conformational changes during viral invasion.