PEG-modified glucose oxidase immobilized on a PVA cryogel membrane for amperometric biosensor applications

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• 作者：Doretti ; Lucio ; Ferrara ; Daniela ; Gattolin ; Paola ; Lora ; Silvano ; Schiavon ; Franco ; Veronese ; Francesco M.
• 关键词：Biosensors ; Glucose oxidase ; PEG-modified glucose oxidase ; PVA cryogel
• 刊名：Talanta
• 出版年：1998
• 出版时间：March, 1998
• 年：1998
• 卷：45
• 期：5
• 页码：891-898
• 全文大小：623 K

文摘

Poly(ethylene glycol)-modified glucose oxidase was immobilized in a poly(vinyl alcohol) cryogel membrane, obtained by a freezing–thawing cyclic process, to obtain a suitable amperometric glucose sensor. The covalent linkage between PEG and GOD molecule improved the physical immobilization of enzyme in the polymeric matrix, by decreasing its loss in time. Sensor behaviour was evaluated electrochemically with a hydrogen peroxide electrode. The glucose content in standard solutions was determined and linear calibration curves in the 5×10⁻⁵–5×10⁻³ mol l⁻¹ range were obtained. The kinetic parameters in the immobilized system were evaluated and analytical characteristics of sensor, including stability and influence of pH and temperature, were determined.