Formation of Cu or Fe thiobarbiturate complexes interfere with the determination of malondialdehyde
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- 作者：Zhou ; Lily ; Sorenson ; John R.J.
- 关键词：Copper ; Iron ; Thiobarbituric acid ; Absorbance ; Fluorescence ; Malondialdehyde
- 刊名：Journal of Inorganic Biochemistry
- 出版年：1998
- 出版时间：December, 1998
- 年：1998
- 卷：72
- 期：3-4
- 页码：217-225
- 全文大小：229 K
文摘
This research was conducted to determine whether or not Cu(II) and Fe(III) form TBA complexes that absorb and fluoresce at the same wavelengths as the bis-TBA-MDA adduct and support a false conclusion that Cu(II) and Fe(III) cause lipid and other tissue component oxidations as evidenced by the apparent formation of the bis-TBA-MBA adduct. Additions of Cu(II) or Fe(III) to Na TBA gave concentration related increases in absorbance at 532 nm, the wavelength of maximum absorbance of the bis-TBA-MDA adduct. These absorbance spectra demonstrate that the addition of aqueous solutions of Na TBA to systems containing added Cu(II) and/or Fe(III) and their butanol extracts will support the false conclusion that Cu(II) and/or Fe(III) caused lipid and other tissue component oxidations. Fluorescence at 553 nm, the maximum for fluorescence of the bis-TBA-MDA adduct, increased as the concentration of Cu(II) increased but decreased with the addition of Fe(III). Fluorescence obtained following the addition of Cu(II) to Na TBA will support the false conclusion that Cu(II) causes lipid and other tissue component oxidations while the lack of fluorescence following the addition of Fe(III) will not support this false conclusion. These studies show that the presence of Cu(II) or Fe(III) in TBA assay systems lead to the specious interpretation that oxidations occurred due to the formation of radical via Cu(II)- or Fe(III)-mediated Fenton chemistry.