The resetting of the circadian rhythm by Prostaglandin J₂ is distinctly phase-dependent

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• 作者：Satoshi Koinuma ; Kazuhiro Yagita ; Atsuko Fujioka ; Naoyuki Takashima ; Toru Takumi ; Yasufumi Shigeyoshi
• 关键词：Circadian rhythm ; Prostaglandin J₂ ; Phase shift ; Per1 ; Dark pulse ; Clock gene
• 刊名：FEBS Letters
• 出版年：2009
• 出版时间：22 January 2009
• 年：2009
• 卷：583
• 期：2
• 页码：413-418
• 全文大小：449 K

文摘

The circadian rhythm can be reset by a variety of substances. Prostaglandin J₂ (PGJ₂) is one such substance and resets the circadian rhythm in fibroblasts. In our current study, we examined the phase-dependent phase shift following PGJ₂ treatment using a real-time luciferase luminescence monitoring system. In the phase response curves, we observed 12 h differences in the times of peaks in comparison with the same analysis for forskolin. Quantification of clock gene mRNAs following PGJ₂ administration additionally revealed a rapid decrease in the Per1, Rev-erbAα and Dbp levels. Our current findings thus suggest that PGJ₂ resets the peripheral circadian clock via a mechanism that is distinct from that used by forskolin (FK).