Npa3/ScGpn1 carboxy-terminal tail is dispensable for cell viability and RNA polymerase II nuclear targeting but critical for microtubule stability and function

详细信息    查看全文

• 作者：Gehenna Guerrero-Serrano^a ; Leonardo Castanedo^a ; Gema R. Cristó ; bal-Mondragó ; n^a ; Javier Montalvo-Arredondo^b ; Lina Riego-Ruí ; z^b ; Alexander DeLuna^c ; Alejandro De Las Peñ ; as^b ; Irene Castañ ; o^b ; Mó ; nica R. Calera^a ; ^{mcalera@ifisica.uaslp.mx} ; Roberto Sá ; nchez-Olea^a ; ^{rsanchez@ifisica.uaslp.mx}
• 关键词：GTPase Gpn1 ; Npa3/ScGpn1 carboxy terminal tail ; Benomyl ; Microtubules ; BIK1 ; Saccharomyces cerevisiae
• 刊名：Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：1864
• 期：3
• 页码：451-462
• 全文大小：2068 K
• 卷排序：1864

文摘

ScGpn1 C-terminal tail is dispensable for viability and RNAPII nuclear targeting. ScGpn1 C-tail controls cell size, mitosis exit, and ScGpn1 and Rtr1 nuclear export. gpn1ΔC cells are hypersensitive to microtubule disruption by benomyl. Microtubule integrity is lost at a sublethal benomyl concentration in gpn1ΔC cells. GPN1 interacts genetically with BIK1, the yeast orthologue of human CLIP-170.