Gene expression profile of cytokines and chemokines in skin lesions from Brazilian Indians with localized cutaneous leishmaniasis

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• 作者：Matheus Fern ; es Costa-Silva ; Luciana In谩cia Gomes ; Olindo Assis Martins-Filho ; Renata Rodrigues-Silva ; Jana铆na de Moura Freire ; Patr铆cia Fl谩via Quaresma ; Marcelo Ant么nio Pascoal-Xavier ; Tiago Ant么nio de Oliveira Mendes ; Rog茅ria Serakides ; Danielle Alves Gomes Zauli ; Ana Carolina Campi-Azevedo ; Maria Norma Melo ; C茅lia Maria Ferreira Gontijo ; Vanessa Peruhype-Magalh茫es ; Andr茅a Teixeira-Carvalho
• 关键词：Cutaneous leishmaniasis ; Localized immune response ; Cytokines ; Chemokines ; Gene expression
• 刊名：Molecular Immunology
• 出版年：February, 2014
• 年：2014
• 卷：57
• 期：2
• 页码：74-85
• 全文大小：2884 K

文摘

Cutaneous leishmaniasis (CL) is a chronic inflammatory disease caused by dermotropic Leishmania species belonging to the Viannia subgenera, with Leishmania (V.) braziliensis considered the main agent in Brazil. After infection, a local inflammatory process is initiated, inducing the expression of several cytokine/chemokine genes. We evaluated the immunity to CL of patients living in the indigenous community Xakriab谩, Minas Gerais state, Brazil, by performing detailed analyses of the mRNA expression of different cytokines and chemokines in CL lesions, considering the time evolution (recent or late). We also studied the profile of the inflammatory infiltrate by histopathological analysis. The histopathological features of recent CL lesions showed an intense inflammatory reaction, characterized by the presence of both mononuclear and polymorphonuclear cells, whereas late CL lesions exhibited a predominance of mononuclear leukocytes. The gene expression of cytokines/chemokines in skin biopsies from the CL group showed higher transcript levels of modulatory (IL10 and TGFB1), anti-inflammatory (IL4), and pro-inflammatory (TNF, IFNG, IL12B, CCL2, CCL3, CCL5, CXCL10) biomarkers in recent lesions than in late lesions. Our findings suggest that differential gene expression of cytokines and chemokines found in skin lesions from CL patients is associated with time evolution of lesions.