Knockdown of Oct4 and Nanog expression inhibits the stemness of pancreatic cancer cells

详细信息    查看全文

• 作者：Yuhua Lu ; Hui Zhu ; Haiyan Shan ; Junjie Lu ; Xu Chang ; Xiaohong Li ; Jingjing Lu ; Xiangjun Fan ; Shajun Zhu ; Yao Wang ; Qingsong Guo ; Lei Wang ; Yan Huang ; Mingyan Zhu ; Zhiwei Wang
• 关键词：Pancreatic cancer ; Cancer stem cells ; Oct4 ; Nanog
• 刊名：Cancer Letters
• 出版年：2013
• 出版时间：28 October, 2013
• 年：2013
• 卷：340
• 期：1
• 页码：113-123
• 全文大小：3282 K

文摘

Pancreatic cancer is notorious for its difficult diagnosis at early stage and poor recurrence-free prognosis. This study aimed to investigate the possible involvement of Oct4 and Nanog in pancreatic cancer. The high expressions of Oct4 and Nanog in human pancreatic cancer tissues were found to indicate a worse prognostic value of patients. The pancreatic cancer stem cells (PCSCs) that isolated from PANC-1 cell line by flow cytometry exhibited high expressions of Oct4 and Nanog. To investigate whether Oct4 and Nanog play crucial role in maintaining the stemness of PCSCs, double knockdown of Oct4 and Nanog demonstrated that Oct4 and Nanog significantly reduced proliferation, migration, invasion, chemoresistance, and tumorigenesis of PCSCs in vitro and in vivo. The altered expression of the genes related to pancreatic carcinogenesis, metastasis, drug resistance and epithelial-mesenchymal transdifferentiation (EMT) might affect the biological characteristics of PCSCs. Our results suggest that Oct4 and Nanog may serve as a potential marker of prognosis and a novel target of therapy for pancreatic cancer.