22 volunteers, total PSA of 4.5–19.3 µg/L, had blood drawn simultaneously. Baseline measurements were performed and samples were stored under various conditions prior to measurements. Freeze–thaw cycles were performed. [-2]proPSA was measured with the p2PSA automated research use only immunoassay on the Access analyzer.
Mean [-2]proPSA increases with clotting time, exceeding 10 % change in recovery after 3 h. In serum, [-2]proPSA values decline over time under investigated storage conditions. Serum samples kept frozen show less than 10 % variation in recoveries over the course of 2 freeze–thaw cycles.
For proper measurement of [-2]proPSA, blood samples should be centrifuged within 3 h of blood draw. Serum may be stored at RT or refrigerated (+ 4 °C) for a maximum of 48 h and should be frozen if stored for a longer period. Two freeze–thaw cycles have no effect on [-2]proPSA stability.