Identification of epigenetically downregulated Tmem70 and Ube2e2 in rat liver after 28-day treatment with hepatocarcinogenic thioacetamide showing gene product downregulation in hepatocellular preneoplastic and neoplastic lesions produced by tumor promotion

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• 作者：Sayaka Mizukami^a ; ^b ; ^{smizuka@cc.tuat.ac.jp} ; Atsunori Yafune^a ; ^{yafune-atsunori@bozo.co.jp} ; Yousuke Watanabe^a ; ^b ; ^{ywatana@cc.tuat.ac.jp} ; Kota Nakajima^a ; ^b ; ^{knakaji@m2.tuat.ac.jp} ; Meilan Jin^c ; ^{meilan0622@swu.edu.cn} ; Toshinori Yoshida^a ; ^{yoshida7@cc.tuat.ac.jp} ; Makoto Shibutani^a ; ^{mshibuta@cc.tuat.ac.jp}
• 关键词：ATP ; adenosine triphosphate ; BNF ; β-naphthoflavone ; CGI ; CpG island ; CT ; threshold cycle ; DEN ; N-diethylnitrosamine ; FB ; fenbendazole ; gDNA ; genomic DNA ; GST-P ; glutathione S-transferase placental form ; HIST1H2AA ; histone cluster 1 H2A family member a ; IP DNA ; immunoprecipitated DNA ; PB ; phenobarbital ; PBO ; piperonyl butoxide ; PCNA ; proliferating cell nuclear antigen ; RT-PCR ; reverse-transcription polymerase chain reaction ; SLK ; STE20-like kinase ; TAA ; thioacetamide ; TMEM70 ; transmembrane protei
• 刊名：Toxicology Letters
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：266
• 期：Complete
• 页码：13-22
• 全文大小：3236 K
• 卷排序：266

文摘

Epigenetically downregulated genes were identified in TAA-treated rat livers. Hist1h2aa, Tmem70, Ube2e2, and Slk showed hypermethylation and mRNA downregulation. Tumor promotion increased downregulation of TMEM70 and UBE2E2 in GST-P+ foci. Downregulation of TMEM70 and UBE2E2 continued to the neoplastic stage. Cell respiration and cell cycling may be carcinogenic targets from early stages.