Progesterone inhibits proliferation and modulates expression of proliferation—Related genes in classical progesterone receptor-negative human BxPC3 pancreatic adenocarcinoma cells

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• 作者：Alexey I. Goncharov^a ; Aitsana A. Maslakova^a ; Anna V. Polikarpova^a ; Elena A. Bulanova^b ; Alexandra A. Guseva^a ; Ivan A. Morozov^c ; Petr M. Rubtsov^c ; Olga V. Smirnova^a ; Tatiana A. Shchelkunova^a ; ^{Schelkunova-t@mail.ru} ; ^{smirnova_ov@mail.ru}
• 关键词：nPRs ; nuclear progesterone receptors ; mPRs ; membrane progesterone receptors ; PGRMC1 ; progesterone receptor membrane component 1 ; GR ; glucocorticoid receptors ; QPCR ; quantitative real-time PCR ; Kd ; the equilibrium dissociation constant ; RBA ; relative binding affinity ; MR ; mineralocorticoid receptors ; Ki67 ; marker of proliferation ; PCNA ; proliferating cell nuclear antigen ; cyclin D1 ; a cyclin-dependent kinases activator ; p21 ; a cyclin-dependent kinase inhibitor 1A ; p27 ; a cyclin-dependent kinase i
• 刊名：The Journal of Steroid Biochemistry and Molecular Biology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：165
• 期：part_PB
• 页码：293-304
• 全文大小：1077 K
• 卷排序：165

文摘

In human pancreatic adenocarcinoma BxPC3 cells, high mPR mRNA levels and the absence of nPR mRNA have been detected. Western blot analysis showed the presence of the mPRα, mPRβ, mPRγ proteins and the absence of nPR protein in BxPC3 cells. In BxPC3 cells, progesterone at high concentration of 20 mM significantly reduced the mRNA levels of proliferation markers Ki67 and PCNA, as well as of cyclin D1. In BxPC3 cells, progesterone at high concentration of 20 μM significantly increased the mRNA levels of cyclin dependent kinase inhibitors p21 and p27. Progesterone at 1 μM and 20 μM concentrations inhibited proliferative activity of BxPC3 cells.