Halorhabdus rudnickae sp. nov., a halophilic archaeon isolated from a salt mine borehole in Poland

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• 作者：Luciana Albuquerque^a ; Magdalena Kowalewicz-Kulbat^b ; Dominika Drzewiecka^c ; Paweł Stączek^d ; Giuseppe d&rsquo ; Auria^e ; ^h ; Ramon Rossell&oacute ; -M&oacute ; ra^f ; Milton S. da Costa^a ; ^g ; ^{milton@ci.uc.pt" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Halorhabdus ; Halorhabdus rudnickae sp. nov ; Haloarchaea
• 刊名：Systematic and Applied Microbiology
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：39
• 期：2
• 页码：100-105
• 全文大小：1076 K

文摘

Two halophilic archaea, designated strains WSM-64^T and WSM-66, were isolated from a sample taken from a borehole in the currently unexploited Barycz mining area belonging to the “Wieliczka” Salt Mine Company, in Poland. Strains are red pigmented and form non-motile cocci that stain Gram-negative. Strains WSM-64^T and WSM-66 showed optimum growth at 40 &deg;C, in 20% NaCl and at pH 6.5–7.5. The strains were facultative anaerobes. The major polar lipids of the two strains were phosphatidylglycerol (PG2), phosphatidylglycerol phosphate methyl ester (PGP-Me) and sulfated diglycosyl diether (S-DGD). Menaquinone MK-8 was the major respiratory quinone. The DNA G+C content of strain WSM-64^T was 61.2 mol% by HPLC method; 61.0 mol% by genome sequencing. Analysis of the almost complete 16S rRNA gene sequence indicated that the strains WSM-64^T and WSM-66 (99.7% identity) represented a member of the genus Halorhabdus in the family Halobacteriaceae. Both strains formed a distinct cluster and were most closely related to Halorhabdus tiamatea SARL4B^T and Halorhabdus utahensis AX-2^T (DSM 12940^T) (95.4% and 95.6%, respectively). ANI values of WSM-64^T with the closest relative type strains were <78.5%. Based on 16S rRNA gene sequence and whole genome analyses, physiological and biochemical characteristics we describe a new species represented by strain WSM-64^T (=DSM 29498^T =CECT 8673^T) for which we propose the name Halorhabdus rudnickae sp. nov.