Liquid-core alginate hydrogel beads loaded with functional compounds of radish by-products by reverse spherification: Optimization by response surface methodology

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• 作者：Fu-Hsuan Tsai^a ; Yutaka Kitamura^b ; ^{kitamura.yutaka.fm@u.tsukuba.ac.jp} ; Mito Kokawa^b
• 关键词：SA ; sodium alginate ; M-block ; β-d-mannuronic acid unit of SA ; G-blocks ; α-l-guluronic acid unit of SA ; CL ; calcium lactate ; RVS ; reverse spherification ; LHB ; liquid-core hydrogel beads ; RBJ ; radish by-product juice ; MAFF ; Ministry of Agriculture ; Forestry and Fisheries (in Japan) ; RSM ; response surface methodology ; MWM ; micro wet milling system ; LCM ; liquid-core material ; LE ; loading efficiency ; TP ; total phenolic compounds ; USP ; United States pharmacopeia ; SGF ; simulated gastric fluid ; SIF ; sim
• 刊名：International Journal of Biological Macromolecules
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：96
• 期：Complete
• 页码：600-610
• 全文大小：1432 K
• 卷排序：96

文摘

Liquid-core hydrogel beads (LHB) were formulated through reverse spherification, by sodium alginate and using calcium lactate (CL) to replace the common calcium source, calcium chloride. The effect of four independent variables: first gelation time (X1), CL concentration in first gelation (X2), secondary gelation time (X3), and CL concentration in secondary gelation (X4), on seven physical properties of LHB: diameter, hardness (Y1), loading efficiency (LE, Y2), release amount of total phenolic compounds (TP) in simulated gastric (Y3) and small intestinal (Y4) fluid, swelling capacity (Y5), and sphericity (Y6), were evaluated. Furthermore, a central composite design with response surface methodology was used for the optimization of LHB properties Y1–Y6, and the importance of the four independent variables to physical properties was analyzed. The diameter of LHB was in the range of 4.17–5.84 mm. The optimal conditions of LHB formulation were first gelation time of 23.99 min, 0.13 M CL in the first gelation, secondary gelation time of 6.04 min, and 0.058 M CL in secondary gelation. The optimized formulation of LHB demonstrated 25.5 N of hardness, 85.67% of LE and 27.38% of TP release in simulated gastric fluid with the small error-values (−2.47 to 2.21%).