Development of a novel target-enriched multiplex PCR (Tem-PCR) assay for simultaneous detection of five foodborne pathogens
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- 作者：Yi-Gang Xua ; b ; Zhong-Mei Liub ; Bai-Qi Zhangc ; Min Qud ; Chun-Sheng Mod ; Jia Luoc ; Su-Long Lib ; ciqlsl@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Tem-PCR ; Foodborne pathogens ; Simultaneous detection
- 刊名：Food Control
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：64
- 期：Complete
- 页码：54-59
- 全文大小：490 K
文摘
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A novel Tem-PCR assay for detection of five foodborne pathogens was developed.
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The assay overcomes the amplification disparity resulting from different primers.
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The assay has been validated in naturally contaminated foods.
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The assay is helpful for developing high-throughput multiplex PCR assay.