Enzyme-catalyzed Michael addition for the synthesis of warfarin and its determination via fluorescence quenching of l-tryptophan
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- 作者：Yusheng Yuan<sup>asup> ; Liu Yang<sup>asup> ; Shaopu Liu<sup>asup> ; Jidong Yang<sup>bsup> ; Hui Zhang<sup>asup> ; Jingjing Yan<sup>asup> ; Xiaoli Hu<sup>asup><sup> ; sup><sup>xiaolihu@swu.edu.cnsup>
- 关键词：Bio-catalysis ; Michael addition ; Porcine pancreas ; Warfarin ; Fluorescence
- 刊名：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
- 出版年：2017
- 出版时间：5 April 2017
- 年：2017
- 卷：176
- 期：Complete
- 页码：183-188
- 全文大小：758 K
- 卷排序：176
文摘
This is the first application of biocatalytic synthesis and fluorescence for the determination of warfarin. The validity was verified by comparing the results with other methods and the detection limit is 0.01 μmol L− 1. The quenching mechanism has been investigated systematically by the Stern–Volmer equation, fluorescence lifetime, UV-visible absorbance spectra.