Identification of nuclear localization signal within goldfish Tgf2 transposase
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- 作者：Xiao-Dan Shen ; Fei Hou ; Jie Chen ; Xia-Yun Jiang ; jiangxy@shou.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Shu-Ming Zou ; smzou@shou.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：NLS ; nuclear localization signal ; hAT ; Hobo/Activator/Tam3 ; Tgf2 ; transposon of goldfish ; Tgf2TPase ; Tgf2 transposase ; L-Tgf2TP ; full-length Tgf2TPase ; Tgf2TP△120N ; N-terminal truncated Tgf2TPase ; Tgf2TP△31C and Tgf2TP△16C ; C-terminal truncated Tgf2TPase ; TEs ; transposable elements ; Tol2 ; transposon of Oryzias latipes ; DDE ; aspartic acid-aspartic acid-glutamic acid ; cDNA ; complementary DNA ; aa ; amino acid(s) ; mRNA ; message RNA ; PCR ; polymerase chain reaction ; pEGFP-C1 ; plasmid containing C
- 刊名：Gene
- 出版年：2016
- 出版时间：15 November 2016
- 年：2016
- 卷：593
- 期：1
- 页码：21-27
- 全文大小：1065 K
文摘
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We investigated a nuclear localization signal (NLS) within Tgf2 transposase.
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A NLS domain was predicted in silico at the C-terminus (656–670 aa).
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NLS domain loss resulted in cytoplasmic but not nuclear protein expression.
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The predicted NLS assists Tgf2 transposase translocation into the nucleus.
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