Fluorescein-based chromo-fluorescent probe for zinc in aqueous solution: Spirolactam ring opened or closed?
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- 作者：Dan WangAuthor Vitae ; Xiaoyan XiangAuthor Vitae ; Xiaolong YangAuthor Vitae ; Xudong WangAuthor Vitae ; Yali GuoAuthor Vitae ; Weisheng LiuAuthor Vitae ; Wenwu Qin ; qinww@lzu.edu.cn" class="auth_mailAuthor Vitae
- 关键词：Bathochromic shifts ; Zinc probe ; Reversible ; Living cells
- 刊名：Sensors and Actuators B: Chemical
- 出版年：1 October 2014
- 年：2014
- 卷：201
- 期：Complete
- 页码：246-254
- 全文大小：2478 K
文摘
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A fluorescein derivative sensor 1 was synthesis and got its crystal structure.
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Sensor 1 shows 50 nm bathochromic shifts and a large increase in the fluorescence intensity upon binding zinc in HEPES buffer solution.
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Sensor 1 does not undergo spirolactam ring opening, whereas ring opening and hydrolysis take place in alkaline pH region.
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Confocal microscopy experiments showed that 1 can be used for detection of Zn2+ levels (∼30 渭M) within living cells.