Top-down and bottom-up analysis of commercial enoxaparins

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• 作者：Xinyue Liu^a ; ^b ; ^{liux22@rpi.edu} ; Kalib St. Ange^b ; ^{stangk2@rpi.edu} ; Lei Lin^b ; ^{Linl5@rpi.edu} ; Fuming Zhang^b ; ^{zhangf2@rpi.edu} ; Lianli Chi^a ; ^{lianlichi@sdu.edu.cn} ; Robert J. Linhardt^b ; ^{linhar@rpi.edu}
• 关键词：LMW heparin ; HILIC-ESI-FTMS ; Top-down ; Bottom-up ; NMR
• 刊名：Journal of Chromatography A
• 出版年：2017
• 出版时间：13 January 2017
• 年：2017
• 卷：1480
• 期：Complete
• 页码：32-40
• 全文大小：2687 K
• 卷排序：1480

文摘

Four types of commercial enoxaparins were analyzed by integrated LC–MS and NMR analysis. Heparinase treatment was used before bottom-up analysis and disaccharide analysis. Intact chain, oligosaccharide, and disaccharide analyses relied on LC–MS. Monosaccharide compositional analysis relied on top-down NMR analysis. Generic enoxaparins and innovator product are similar, differences are observed due to parent heparin and process conditions.