Cholinergic neurons in the basal forebrain of aged female mice

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• 作者：Kalesnykas ; Giedrius ; Puoliv??li ; Jukka ; Sirvi?? ; Jouni ; Miettinen ; Riitta
• 关键词：Neurotransmitters ; modulators ; transporters ; and receptors ; Regional localization of receptors and transmitters ; Aging ; Alzheimer's disease ; Cell counting ; Estrogen ; Post-menopause ; Stereology
• 刊名：Brain Research
• 出版年：2004
• 出版时间：October 1, 2004
• 年：2004
• 卷：1022
• 期：1-2
• 页码：148-156
• 全文大小：398 K

文摘

Aging is associated with at least down-regulation of several cellular functions and diminished responsiveness to internal and external signals, and possibly with direct cell death. Consequently, pharmacological manipulations may be less effective in aged than in young organisms. In the present study, we investigated whether the basal forebrain cholinergic neurons and the estrogen receptor alpha (ER??) which they contain respond to changes in estrogen availability in aged female mice. The mice were sham-operated, ovariectomized, or ovariectomized and treated with 17??-estradiol at the age of 18 months. Three months later, the mice were perfused and brain sections were double immunostained for choline acetyltransferase (ChAT) and ER??. Cell counting with a stereological method revealed that changes in the estrogen level have no effect on the total number of ChAT-immunoreactive (ir) neurons in the basal forebrain. However, the percentage of ChAT-ir neurons containing ER??-ir was higher in the ovariectomized mice than in the sham-operated or estrogen-treated mice. This was specific for the medial septum and vertical diagonal band of Broca. The findings indicate that even at old age the ER??s in cholinergic cells are able to respond to changes in estrogen levels, though in a region-specific manner. This is naturally important for studies aiming to develop therapies for the elderly.