Down-regulation of CatSper1 channel in epididymal spermatozoa contributes to the pathogenesis of asthenozoospermia, whereas up-regulation of the channel by Sheng-Jing-San treatment improves the sperm motility of asthenozoospermia in rats

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• 作者：Ya-Nan Wang ; M.S.^a ; ^b ; Bo Wang ; M.S.^a ; ^b ; Ming Liang ; M.S.^b ; Cai-Yan Han ; M.S.^b ; Bin Zhang ; B.S.^b ; Jie Cai ; M.S.^a ; Wei Sun ; B.S.^b ; ^{sunwei996@126.com} ; Guo-Gang Xing ; M.D. ; Ph.D.^a ; ^c ; ^{ggxing@bjmu.edu.cn}
• 关键词：Asthenozoospermia ; male infertility ; CatSper1 channel ; cyclophosphamide ; Sheng-Jing-San recipe
• 刊名：Fertility and Sterility
• 出版年：2013
• 出版时间：February, 2013
• 年：2013
• 卷：99
• 期：2
• 页码：579-587
• 全文大小：593 K

文摘

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Objective

To determine the expression of CatSper1 channel in epididymal spermatozoa in a rat model of asthenozoospermia, induced by cyclophosphamide (CP), and further examine the effects of soluble granules of Sheng-Jing-San (SJS), a traditional Chinese medicine recipe, on CatSper1 expression and sperm motility in the CP-induced asthenozoospermic rats.

Design

Placebo-controlled, randomized trial.

Setting

Neuroscience Research Institute, Peking University, China.

Animal(s)

Sexually mature male Sprague-Dawley rats (n = 60).

Intervention(s)

In the CP group, CP at the dose of 35 mg/kg intraperitoneally injected into rats once a day for 7 days; in the normal saline (NS) group, 0.9 % saline solution was injected as control.

Main Outcome Measure(s)

Sperm motility and count were evaluated by computer-assisted sperm assay (CASA); protein and mRNA expression of CatSper1 channel in epididymal spermatozoa was determined by Western blotting and quantitative real-time RT-PCR, respectively.

Result(s)

The rats were randomly divided into five groups with 12 rats in each group: CP, normal saline (NS), CP + SJS, CP + NS, and treatment na?ve. In the CP + SJS group, after the last injection of CP, SJS at a dose of 30 mg/kg was intragastrically administrated to rats once a day for 14 days; in CP + NS group, saline solution instead of SJS was administrated as control. In the treatment na?ve group, rats were normally fed for 21 days as controls. We found a statistically significant reduction of the CatSper1 channel, which is associated with an impairment of sperm motility in the epididymal spermatozoa of CP-induced asthenozoospermic rats. Soluble granules of SJS could dramatically restore the CP-induced down-regulation of CatSper1 in epididymal spermatozoa, which greatly improved the sperm motility in the asthenozoospermic rats.

Conclusion(s)

Down-regulation of the CatSper1 channel in epididymal spermatozoa likely contributes to the pathogenesis of asthenozoospermia, whereas up-regulation of the channel by SJS improves sperm motility and thus can be used as an effective therapeutic strategy for the treatment of male infertility diagnosed with asthenozoospermia.