Quantitative determination of major capsaicinoids in serum by ELISA and time-resolved fluorescent immunoassay based on monoclonal antibodies
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- 作者：Qingqing Yanga ; b ; c ; d ; Jianguo Zhua ; b ; c ; d ; Fei Maa ; b ; c ; e ; Peiwu Lia ; b ; c ; d ; e ; peiwuli@oilcrops.cn" class="auth_mail" title="E-mail the corresponding author ; Liangxiao Zhanga ; d ; e ; Wen Zhanga ; b ; e ; Xiaoxia Dinga ; b ; d ; e ; Qi Zhanga ; c ; d ; zhangqi521x@126.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Capsaicin ; Capsaicinoids ; Monoclonal antibody ; Indirect competitive enzyme-linked immunosorbent assay (icELISA) ; Time-resolved fluorescent immunochromatographic assay (TRFICA)
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 July 2016
- 年：2016
- 卷：81
- 期：Complete
- 页码：229-235
- 全文大小：1309 K
文摘
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Novel monoclonal antibodies against deterrent capsaicinoids were achieved.
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A competitive indirect ELISA (icELISA) was established for capsaicinoids detection.
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A time-resolved fluorescent ICA (TRFICA) was developed for capsaicinoids detection.
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The icELISA and TRFICA performed rapidly assay for major capsaicinoids in serum.