- 作者：Catherine A. Brennan ; catherine.brennan@abbott.com ; Julie Yamaguchi ; Ana Vallari ; Priscilla Swanson ; John R. Hackett Jr
- 关键词：HIV Ag/Ab combination assay ; p24 antigen ; HIV-1 viral load ; Acute HIV infection
- 刊名：Journal of Clinical Virology
- 出版年：2013
- 出版时间：June, 2013
- 年：2013
- 卷：57
- 期：2
- 页码：169-172
- 全文大小：421 K
Background
HIV antigen/antibody (Ag/Ab) combination assays represent a significant advancement in assays used for diagnosing HIV infection based on their ability to detect acute and chronic infections. During acute HIV infection (AHI), detection depends on assay sensitivity for p24 Ag.Objective
To directly compare the Ag sensitivity of the ARCHITECT? HIV Ag/Ab Combo assay to RNA viral load using cell culture supernatants of virus isolates. HIV-1 isolates allow correlation in the total absence of an antibody response to infection and across genetically diverse HIV-1 group M strains.
Methods
Thirty-five HIV-1 isolates comprising subtypes A-D, F and G, CRF01_AE, CRF02_AG, and unique recombinant forms were evaluated. Cell-free culture supernatant for each isolate was diluted to four levels and tested in the HIV Combo assay to determine a signal to cutoff ratio and the RealTime? HIV-1 assay to quantify RNA. The RNA copies/mL at the HIV Combo assay cutoff was determined.
Results
The median RNA copies/mL at the HIV Combo assay cutoff was 57,900 for individual virus isolates (range 26,440-102,400). A single plot of all the data gave a value of 58,500 RNA copies/mL. An analysis of data published for acute HIV infection in human subjects gave a similar result; HIV Combo detected 97 % of AHIs with RNA copies/mL > 30,700.
Conclusions
Based on analysis of virus isolates, the ARCHITECT HIV Combo assay can detect p24 Ag when RNA is above approximately 58,000 copies/mL. The correlation of viral load and Ag sensitivity was consistent across genetically diverse HIV-1 group M strains.