Wiring of pyranose dehydrogenase with osmium polymers of different redox potentials

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• 作者：Muhammad Nadeem Zafar ; Federico Tasca ; Susan Bol ; Magdalena Kujawa ; Ilabahen Patel ; Clemens K. Peterbauer ; Dó ; nal Leech ; Lo Gorton
• 关键词：Biosensor ; Biofuel anode ; Chemically modified electrode ; Sugar oxidising enzyme ; Graphite
• 刊名：Bioelectrochemistry
• 出版年：2010
• 出版时间：November 2010
• 年：2010
• 卷：80
• 期：1
• 页码：38-42
• 全文大小：608 K

文摘

In this study, five different flexible osmium based redox polymers were investigated for their ability to efficiently “wire” the oxidoreductase pyranose dehydrogenase (PDH, EC 1.1.99.29) from Agaricus meleagris, on graphite electrodes for possible applications in biofuel cells. A series of newly synthesised osmium based redox polymers covering the potential range between − 270 and + 160 mV vs. AgAgCl (0.1 M KCl) was used. The performance of the redox polymers for enzyme wiring was investigated using glucose as substrate. The optimal operational conditions such as pH and potential were investigated.