Alteration of working memory but not in anxiety or stress response in p300/CBP associated factor (PCAF) histone acetylase knockout mice bred on a C57BL/6 background

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• 作者：Florian Duclot ; Chantal Jacquet ; Cé ; line Gongora ; Tangui Maurice
• 关键词：Histone acetyltransferase PCAF ; Short-term memory ; Spatial memory ; Behavioural despair ; Anxiety
• 刊名：Neuroscience Letters
• 出版年：2010
• 出版时间：21 May 2010
• 年：2010
• 卷：475
• 期：3
• 页码：179-183
• 全文大小：368 K

文摘

P300/CBP associated factor (PCAF) acts as an acetyltransferase that acetylates specific lysine residues in histones, thereby remodelling chromatin structure. The possible involvement of PCAF in learning and memory processes or mood disorders was recently assessed by characterizing the behavioural phenotype of PCAF KO mice bred on a CD1 background and revealed short-term memory deficits that evolved with age towards long-term memory alteration and an exaggerated response to stress [10]. PCAF KO mice have been backcrossed on a C57BL/6j strain for 15 generations and we report here the first data regarding their behavioural phenotype. PCAF KO mice bred on a C57 background showed short-term memory deficits in terms of decreased spontaneous alternation and absence of acquisition of a daily changing platform position in the water-maze. Acquisition of a fixed platform location or passive avoidance response was preserved. PCAF KO mice showed no difference with WT C57BL/6j controls in their performances in the forced swimming and light/dark exploration box, suggesting no particular phenotype on anxiety and stress responses. We therefore evidenced marked phenotypic differences in PCAF KO mice depending on the genetic background strain confirming that PCAF histone acetyltransferase is involved lifelong in the chromatin remodelling necessary for memory formation but differentially involved in anxiety and response to stress.