Selective carboxylate production by controlling hydrogen, carbon dioxide and substrate concentrations in mixed culture fermentation

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• 作者：D. Arslan^a ; ^b ; ^{doga.arslan@vito.be} ; K.J.J. Steinbusch^b ; L. Diels^a ; H. De Wever^a ; H.V.M. Hamelers^b ; C.J.N. Buisman^b
• 关键词：Hydrogen ; Carbon dioxide ; Headspace ; Carboxylates ; Organic waste substrate
• 刊名：Bioresource Technology
• 出版年：2013
• 出版时间：May, 2013
• 年：2013
• 卷：136
• 期：Complete
• 页码：452-460
• 全文大小：1050 K

文摘

This research demonstrated the selective production of n-butyrate from mixed culture by applying 2 bar carbon dioxide into the headspace of batch fermenters or by increasing the initial substrate concentration. The effect of increasing initial substrate concentration was investigated at 8, 13.5 and 23 g COD/L with potato processing waste stream. Within 1 week of incubation, n-butyrate fraction selectively increased up to 83 % by applying 2 bar hydrogen or 78 % by applying carbon dioxide into the headspace whereas it was only 59 % in the control reactor. Although the fraction of n-butyrate was elevated, the concentration remained lower than in the control. Both the highest concentration and fraction of n-butyrate were observed under the highest initial substrate concentration without headspace addition. The concentration was 10 g COD/L with 73 % fraction. The operational conditions obtained from batch experiments for selective n-butyrate production were validated in a continuous process.