Comparison of three screening methods to select mixed-microbial inoculum for mixed-acid fermentations

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• 作者：Kristina W. Golub^a ; ^{golubkw@gmail.com} ; Andrea K. Forrest^a ; Melinda E. Wales^a ; Amy Jo M. Hammett^b ; Julia L. Cope^b ; Heather H. Wilkinson^b ; Mark T. Holtzapple^a
• 关键词：Carboxylate platform ; MixAlco&trade ; process ; Bioscreening ; Bioprospecting ; Mixed-acid fermentation
• 刊名：Bioresource Technology
• 出版年：2013
• 出版时间：February, 2013
• 年：2013
• 卷：130
• 期：Complete
• 页码：739-749
• 全文大小：638 K

文摘

Using a mixed culture of microorganisms, the carboxylate platform converts biomass into hydrocarbons and chemicals. To develop a method that identifies the highest performing inoculum for carboxylate fermentations, five bacterial communities were screened and ranked by three fermentation performance tests: (1) 30-day batch screen, (2) 28-day continuum particle distribution model (CPDM), and (3) 5-month continuous countercurrent fermentation trains. To screen numerous inocula sources, these tests were used sequentially in an aseptic environment. For the batch-fermentation screen, Inoculum 1 achieved the highest conversion. For the CPDM evaluation, the operating map for Inoculum 1 had the highest performance. For the continuous countercurrent fermentation, the train resulting from Inoculum 1 was among the best performers. This study suggests that the three screens are a useful and predictive method for choosing optimal inocula sources. The bacterial community with optimal performance in these three screens could be considered for use in commercial-scale fermentations.