A simple and efficient method for releasing DNA from Dermatophilus congolensis

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• 作者：Makinde ; A.A. ; Gyles ; C.L.
• 关键词：DNA extraction ; Recovery of total genomic DNA ; Dermatophilus congolensis
• 刊名：Journal of Microbiological Methods
• 出版年：1998
• 出版时间：August, 1998
• 年：1998
• 卷：33
• 期：3
• 页码：197-202
• 全文大小：465 K

文摘

A modification of a combination of various techniques for extracting DNA from Actinomycetes and other Gram-positive bacteria was adapted for releasing DNA from Dermatophilus congolensis. This was based on pre-treatment of whole cells of Dermatophilus congolensis with acetone followed by further treatment in a solution containing achromopeptidase, lysozyme and ethylenediamine tetracetic acid (EDTA) before the final exposure to sodium dodocyl sulphate (SDS) for complete lysis. This protocol resulted in good lysis leading to sufficient yield and satisfactory recovery of chromosomal DNA from Dermatophilus congolensis. Agarose gel electrophoresis of DNA revealed one to seven discrete bands of extrachromosomal DNA which were observed in six isolates. This procedure which provides recovery of good quality total genomic DNA should pave the way for studies involving genetic analysis of Dermatophilus congolensis including genotypic typing.