Effect of Praeruptorin C on 3-nitropropionic acid induced Huntington’s disease-like symptoms in mice

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• 作者：Lu Wang^a ; ¹ ; Jing Wang^b ; ¹ ; Le Yang^a ; Shi-meng Zhou^a ; ¹ ; Shao-yu Guan^c ; Liu-kun Yang^c ; Qi-xin Shi^c ; Ming-Gao Zhao^a ; Qi Yang^a ; ^{yangqifmmu@126.com}
• 关键词：3-NP ; 3-nitropropionic acid ; Pra-C ; Praeruptorin C ; OFT ; open field test ; FST ; forced swimming test ; TST ; tail suspension test ; MSNs ; medium spiny neurons ; BDNF ; brain derived neurotrophic factor ; HPLC ; high performance liquid chromatography ; GABA ; γ-aminobutyric acid ; DNFB ; 2 ; 4-dinitrofluorobenzene
• 刊名：Biomedicine & Pharmacotherapy
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：86
• 期：Complete
• 页码：81-87
• 全文大小：1619 K
• 卷排序：86

文摘

Huntington’s disease (HD) is an autosomal dominant inherited disease characterized by movement, psychiatric, and cognitive disorders. Previous research suggests that Praeruptorin C (Pra-C), an effective component in the root of Peucedanum praeruptorum dunn, a traditional Chinese medicine, may function in neuroprotection. The present study was conducted to evaluate the effectiveness of Pra-C in the treatment of HD-like symptoms in a 3-nitropropionic acid (3-NP) mouse model, and to explore the possible mechanism of the drug’s activity. We treated 3-NP-injected mice with two different doses of Pra-C (1.5 and 3.0 mg/kg) for 3 days. Motor behavior was tested using the open field test (OFT) and rotarod test, while psychiatric symptoms were tested using the forced swimming test (FST) and tail suspension test (TST). We found that Pra-C alleviated the motor deficits and depression-like behavior in the 3-NP-treated mice, and protected neurons from excitotoxicity. Western blot analysis revealed that Pra-C upregulated BDNF, DARPP32, and huntingtin protein in the striatum of 3-NP mice. These results taken together suggest that Pra-C may have therapeutic potential with respect to the movement, psychiatric, and cognitive symptoms of HD.